| 1. | The immediate transcript of a trna gene is known as precursor trna .
TRNA基因的直接转录物叫做tRNA前体。 |
| 2. | The immediate transcript of a trna gene is known as precursor trna
Trna基因的直接转录物叫做trna前体。 |
| 3. | We have also analyzed the nucleotide and deduced amino acid sequences of mitochondrial genes , the formation cause of a pseudogene for trnamel , and proposed the secondary structures of 22 trna genes
我们还分析了基因组的碱基和氨基酸的组成、 trna ~ ( met )假基因的成因，推测了22个trna的二级结构。 |
| 4. | Our previous work identified delayed mutation occurred at target supf trna gene in plasmid transfected into cells pretreated with low concentration of alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng )
在收回的质粒上我们检测到了延迟发生的、高于对照5倍以上的突变。由于w在细胞中的半寿期仅为1 |
| 5. | Direct damage on supf trna gene can be neglected because half - life of mnng is 1 . 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours . therefore the mutagenesis is not lesion directed
20m的mnng经洗涤后的极微量残留经过10 ? 20多个半衰期的降解后，己不足攻击转人的qdna分子，因此这种突变显然是发生在mnng直接攻击部位以外的正常碱基上。 |
| 6. | Scolopendra multilan is a common species belonging to scolopendromorpha , chilopoda . the sequence of scolopendra multilan mtdna determined is 11700 bp in length , about 70 % of the complete sequence . the sequenced portion contains 2 rrna genes , 1 a + t - rich region , 8 protein - coding genes and 18 trna genes
本文对少棘蜈蚣的线粒体基因组进行了研究，已经测定的序列长11700bp ，约为全长序列的70 ，包含了2个rrna基因、 1个at富含区、 8个蛋白基因、 18个trna基因。 |
| 7. | The partial sequences of the mitochondrial co i , co ii and trna gene of the adults and larvae of 4 species from lepidostomatidae and the adults and larvae of apsilochorema unculatum and a . hwangi were sequenced and compared in this study . in the sequences obtained ( 1718 ~ 2239bp ) , a % + t % was about 69 . 2 % , 267 nucleotide sites were substituted in apsilochorema and 266 nucleotide sites were substituted in lepidostomatidae . at bias in the third codon position site was much higher than the other two sites , reaching about 81 . 9 %
{的比对分析表明:在coi区的一45一个比对位点中，有1260个保守位点， 191个变异位点，其中98个转换位点， 93个颠换位点;发生在密码子第三、第一和第二位点的变异分别为78 % 、 18 %和4 % ;种内序列歧异度为o一0 . 9 % ，而种间为一4 %一24 % 。 |
| 8. | In our laboratory , a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0 . 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0 . 2m mnng for 2 . 5h , then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture . we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells . this kind of mutation did not occur immediately after mnng exposure
我们实验室曾用一特殊的突变检测系统，直接证明dna损伤剂可在哺乳动物细胞诱发非定标性突变：首先用低浓度( 0 . 2 m )的短寿烷化剂mnng (半寿期为1 . 1hr )处理细胞2 . 5h后，继续培养24h ，将重组有用作突变检测的靶基因supftrna基因的穿梭质粒pz189转入细胞复制，发现在未受致癌物直接攻击的穿梭质粒中有较自发突变率高5倍以上的靶基因突变。 |
| 9. | A four - gene block , a rwo - trna gene cluster and six single trna genes involved in the rearrangement , the second gene rearrangement type of mitochondrial dna reported for any pancrustacea arthropod . comparisons of mitochondrial gene arrangements of decapod suggested that rearrangement of the four gene - block found in eriocheir is informative for high level phylogenetic study of decapod
发生重排的基因包括一个4基因块的重排、 1个2基因的trna基因簇的重排和6个单一trna基因的重排，形成泛甲壳类线粒体dna的另一种基因重排类型。 |
